Hydrolysis Behaviour of Lipase from Pseudomonas Cepacia Encapsulated in Silica Aerogels with Different Characteristics
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منابع مشابه
Characteristics of a Monoacylglycerol Lipase Isolated from Pseudomonas sp. LP7315 -Hydrolysis and Synthesis of Monoglycerides
A monoacylglycerollipase (MGL) was purified from Pseudomonas sp. LP7315 by ammonium sulfate precipitation, anion-exchange chromatography, and preparative electrophoresis. The purified enzyme was homogeneous on an SDS-polyacrylamide gel with a molecular mass of 59 kDa. Its hydrolytic activity was confirmed to be specific for monoglycerides: the enzyme did not hydrolyze diand triglycerides. MGL w...
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To investigate the enantioselectivity of Pseudomonas cepacia lipase, inhibition studies were performed with Sc- and Rc-(Rp,Sp)-1,2-dialkylcarbamoylglycero-3-O-p-nitrophenyl alkylphosphonates of different alkyl chain lengths. P. cepacia lipase was most rapidly inactivated by Rc-(Rp,Sp)-1,2-dioctylcarbamoylglycero-3-O-p-nitrophenyl octylphosphonate (Rc-trioctyl) with an inactivation half-time of ...
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Lipase from Pseudomonas cepacia was covalently immobilized on crystalline silicon, porous silicon and silicon nitride surfaces. The various stages of immobilization were characterized using FTIR (Fourier transform infrared) spectroscopy. The surface topography of the enzyme immobilized surfaces was investigated using scanning electron microscopy (SEM). The quantity of the immobilized active enz...
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A continuous process for the glycerolysis of soybean oil with pure and crude glycerol, the co-productfrom the rransesteriiication of soybean oil was invesligated i n a pilot plan(. Thc placess was equipped with a motionless and a high-shear mixer. The experimental studies explored the effectc of variations i n mixing inlensiLy, remperature, reactant flow rates, and reactant stoichiometry on the...
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ژورنال
عنوان ژورنال: Macromolecular Bioscience
سال: 2001
ISSN: 1616-5187,1616-5195
DOI: 10.1002/1616-5195(20010601)1:4<119::aid-mabi119>3.3.co;2-r